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X-WR-RELCALID:www_embl_org_seminar_9039.ics
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DTSTAMP:20240328T232417Z
LOCATION:Small Operon\, EMBL Heidelberg
Meyerhofstraße 1
69117 Heid
elberg
Germany
SUMMARY;LANGUAGE=en-us:Harald Dibowski/ Martjin Dankers: Investigating the
function of proteins using a dynamic peptide microarray platform in signal
transduction\, cancer and biomarker research
DESCRIPTION:Company Representative\nSpeaker: Harald Dibowski/ Martjin Danke
rs\nInstitution: Dichrom GmbH Germany / PamGene Netherland\, NOT SPECIFIE
D\nHost: Rainer Pepperkok\nAbstract: Kinases play an essential role in (ca
ncer) biology\, and many of the current drugs are kinase inhibitors. Pepti
de arrays constructed with phosphorylation substrates (up to 256) provide
kinase activity profiles from small tissue samples\, and allow inhibitor p
rofiling. New compounds can be compared to established drugs\, on- and off
-target effects ascertained and kinase activity patterns studied as biomar
kers in pre-clinical and clinical drug development.
This talk prese
nts a novel multiplex kinase assay on a 3 dimensional flow-through microar
ray platform where kinases in complex samples as well as compounds are tes
ted against a panel of peptides\, representing known kinase substrates. Th
is multiplex assay is performed on a fully automated microarray system\, w
hich generates kinetic read-out curves in less than 1 hour\, allowing simu
ltaneous kinase inhibitor potency/IC50\, selectivity\, potency and mechani
sm of action assessments.
In addition\, we will present the latest
results obtained in the application of this novel platform in biomarker di
scovery strategies. This approach is based on measuring kinase activities
in extracts of tissues (including blood\, e.g. PBMCs) and cells. Discovery
of markers at this enzymatic level\, i.e. at the biological level many of
the new targeted drug therapies intervene\, is different from many other s
trategies where DNA mutations/amplification\, RNA or protein levels/modifi
cations are the source of investigation. This activity-based approach is e
nabled by these dynamic peptide microarrays. Examples of applications of b
oth tyrosine and serine/threonine kinase activity profiling in e.g. signal
transduction research\, cell line to animal model comparisons and drug res
ponse predictions will be discussed.\n
DTSTART:20131216T100000Z
LAST-MODIFIED:20240328T232417Z
DURATION:PT1H
UID:www.embl.org/9039
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